![]() ![]() CIS analysis was performed using the reduced scale parameter (5k) in CIMPL. Braf in the colon), top transposon driver genes (e.g., Prdm16, Mecom, and Erg in the AML screen) or transcriptomic subtypes as described in this study (ETP-like vs. Screens were separated according genetic backgrounds (e.g., activated Kras vs. PiggyBac transposon screens were performed in multiple different genetic contexts and organs, related to Figure 5A ATP1 and ATP2 mouse lines were used to induce solid and hematopoietic cancers, respectively. Chr, chromosome #, number enh, enhancer dbSE, dbSuperEnhancer database ChrAccess.incr, chromatin accessibility increase PC, protein coding nPC, non-protein coding. ![]() For intronic CISs, the addition “distant” is added, if the intronic RE is connected to a distant gene. Manual evaluation was used to correct the type in the case of multiple overlaps and to annotate the target genes in the case where multiple genes are reported. In addition, the ARCIS RE score and the CIS type as annotated by ARCIS are shown. Selected output columns of the ARCIS annotation framework are shown: chromHMM output selected for active and/or weak enhancers (compare Figure S5), exons per overlapping transcript in the CIS region, sum of all Hi-C connections in T cell development with origin in the CIS region, the distance to the next super-enhancer from the dbSuper database for thymus tissue, regions of increasing chromatin access in development (compare Hu et al. Besides these large PC CISs, CIS size ranges from 5 to 20 kb. For large protein-coding CISs, CISs are merged to one large CIS by the algorithm (e.g., Ikzf1). CISs were identified using s scale parameter of 5k and according to the RE score. ![]() ARCIS output and manual annotation for Top537 CIS regions, related to Figures 2 and S6 List of common insertion sites (CISs) identified by CIMPL (common insertion site mapping platform) analysis using non-redundant insertions with a read coverage ≥4 (n = 179,075) from 51 Rosa26 PB/+ ATP2 mice as an input. ![]()
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